Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Nuclei: Types of Nuclear Relaxation01:28

Atomic Nuclei: Types of Nuclear Relaxation

Nuclear relaxation restores the equilibrium population imbalance and can occur via spin–lattice or spin–spin mechanisms, which are first-order exponential decay processes.
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers energy to a nearby...
Atomic Nuclei: Nuclear Relaxation Processes01:23

Atomic Nuclei: Nuclear Relaxation Processes

In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis,  the precessing magnetic moments are randomly oriented around the z-axis. This...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inherent MS-cleavability of diazirine photo-cross-links enables residue-level structural analysis.

Nature communications·2026
Same author

Supramolecular Modulation of Photoinduced Charge Transfer: Tuning Between Tunneling and Incoherent Hopping.

Angewandte Chemie (International ed. in English)·2026
Same author

Mesenchymal stem cell-derived exosomes promote scalp rejuvenation through type XVII collagen regulation via the miR-21-5p/DKK2/Wnt pathway.

Frontiers in cell and developmental biology·2026
Same author

Dynamic Interfacial Evolution and Miscibility of CO<sub>2</sub>-Alkane Systems: The Role of Chain Length.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Unveiling the Dynamic Ionic Interactions at the Single-Molecule Resolution.

Journal of the American Chemical Society·2026
Same author

The Impact of Real-Time Data Analytics on Infection Prevention and Control Practices in Ophthalmology: A Nursing Perspective on Patient Outcomes and Cost-Effectiveness.

Nursing & health sciences·2026

Related Experiment Video

Updated: Jul 11, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

Elucidating transient macromolecular interactions using paramagnetic relaxation enhancement.

G Marius Clore1, Chun Tang, Junji Iwahara

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA. mariusc@intra.niddk.nih.gov

Current Opinion in Structural Biology
|October 5, 2007
PubMed
Summary

Paramagnetic relaxation enhancement (PRE) significantly improves Nuclear Magnetic Resonance (NMR) structure determination accuracy. PRE also reveals dynamic processes in macromolecular complex formation and protein-DNA interactions.

More Related Videos

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Related Experiment Videos

Last Updated: Jul 11, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Paramagnetic relaxation enhancement (PRE) is an NMR spectroscopy technique.
  • PRE is valuable for studying macromolecular structure and dynamics.

Purpose of the Study:

  • To present recent advances in using PRE for structure refinement and analyzing dynamic processes in macromolecular complex formation.
  • To demonstrate PRE's utility in enhancing the accuracy and reliability of NMR structure determinations.
  • To explore PRE's application in characterizing protein-DNA and protein-protein interactions.

Main Methods:

  • Utilizing PRE in the slow exchange regime for NMR structure refinement.
  • Employing an ensemble representation of the paramagnetic center.
  • Applying a model-free extension of the Solomon-Bloembergen equations.
  • Analyzing PRE data in the fast exchange regime to study dynamic processes.

Main Results:

  • PRE significantly increases the reliability and accuracy of NMR structure determinations, as shown with the SRY/DNA complex.
  • PRE provides insights into dynamic processes, including transient intermediate species in the fast exchange regime.
  • PRE characterizes dynamic nonspecific binding of proteins to DNA.
  • PRE demonstrates that transcription factors use both intramolecular and intermolecular mechanisms to locate DNA targets.
  • PRE detects and visualizes transient encounter complexes in protein-protein association.

Conclusions:

  • PRE is a powerful tool for refining macromolecular structures and analyzing complex formation dynamics.
  • PRE offers detailed insights into molecular recognition and binding mechanisms.
  • PRE advances the study of transient interactions and dynamic processes in biological systems.