Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electronic detectors for electron microscopy.

A R Faruqi1, R Henderson

  • 1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK. arf@mrc-lmb.cam.ac.uk

Current Opinion in Structural Biology
|October 5, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Physical review letters·2026
Same author

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Physical review letters·2026
Same author

Targeted degradation of CBP/p300 by CBPD-409 exhibits robust anti-myeloma activity.

Leukemia·2026
Same author

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

Physical review letters·2026
Same author

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Physical review letters·2026
Same author

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

Physical review letters·2026
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

New direct electron detectors are needed for electron cryo-microscopy (cryoEM) to overcome the resolution limits of current CCD detectors. This review discusses hybrid pixel detectors (HPDs) and monolithic active pixel sensors (MAPS) for improved cryoEM imaging.

Area of Science:

  • Structural Biology
  • Biophysics
  • Materials Science

Background:

  • Electron cryo-microscopy (cryoEM) is increasingly popular for analyzing large biological molecules.
  • Current CCD detectors have limitations in spatial resolution due to light scattering.
  • Direct electron detection offers a potential solution to improve cryoEM performance.

Purpose of the Study:

  • To review and discuss new direct electron detector technologies for cryoEM.
  • To evaluate these detectors based on parameters critical for cryoEM applications.
  • To address the need for improved detectors for rapid and efficient cryoEM readout.

Main Methods:

  • Discussion of hybrid pixel detectors (HPDs) with separate sensor and readout components.
  • Analysis of monolithic active pixel sensors (MAPS) with integrated sensor and readout.

Related Experiment Videos

  • Focus on key performance metrics: detective quantum efficiency (DQE), modulation transfer function (MTF), radiation hardness, readout speed, signal-to-noise ratio (SNR), and pixel count.

    • Main Results:

    • Direct detection methods avoid the light conversion step inherent in CCDs, potentially improving resolution.
    • HPDs and MAPS represent two promising direct detection approaches.
    • Performance evaluation will guide the selection of optimal detectors for cryoEM.

    Conclusions:

    • Direct electron detectors are essential for advancing cryoEM capabilities.
    • HPDs and MAPS offer distinct advantages and require further investigation for cryoEM applications.
    • Optimized detectors will enhance the structural analysis of biological macromolecules.