Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A New Hypothesis of the Origin of Life ─ Did eukaryotes evolve from the "<i>tamago</i> of life"?

[Kagaku] [Science]·2026
Same author

Cutaneous human papillomavirus E6 impairs the cGAS-STING pathway.

mSphere·2026
Same author

The eIF5-mimic protein 5MP1: a regulator of translation stringency and a multifaceted oncogene.

Journal of biochemistry·2026
Same author

eIF3g binding to GUCG boxes located in mRNA coding regions enhances translation of mild heat shock response genes in the yeast Saccharomyces cerevisiae.

Nucleic acids research·2026
Same author

Genome-wide Survey of Ribosome Collision.

Cell reports·2025
Same author

Layer-controlled continuous MoS<sub>2</sub> growth using a spin-coatable metal precursor buffer.

Nanoscale·2025

Related Experiment Video

Updated: Jul 11, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Yeast phenotypic assays on translational control.

Bumjun Lee1, Tsuyoshi Udagawa, Chingakham Ranjit Singh

  • 1Molecular, Cellular, and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, Kansas, USA.

Methods in Enzymology
|October 5, 2007
PubMed
Summary

Phenotypic assays in yeast reveal how mutations affect translation initiation and gene expression. These studies provide insights into start codon selection and the regulation of protein synthesis.

More Related Videos

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
10:27

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

Published on: July 18, 2011

Related Experiment Videos

Last Updated: Jul 11, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
10:27

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

Published on: July 18, 2011

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Translation is a fundamental biological process crucial for protein synthesis.
  • Understanding translation regulation is key to deciphering gene expression control.
  • Yeast Saccharomyces cerevisiae serves as a powerful model eukaryote for studying conserved cellular mechanisms.

Purpose of the Study:

  • To describe phenotypic assays for analyzing specific and general aspects of translation in yeast.
  • To investigate the impact of mutations on start codon selection stringency.
  • To explore the regulation of Gcn4p translation initiation in response to amino acid availability.

Main Methods:

  • Utilizing his4(-) or his4-lacZ alleles to assess start codon selection.
  • Employing mutations affecting the eIF2/GTP/Met-tRNA(i)(Met) complex and 40S subunit binding.
  • Using amino acid analogs and enzyme inhibitors to study translational phenotypes.
  • Applying polysome profiling to confirm translation defects.

Main Results:

  • Mutations relaxing start codon selection confer His(+) or Sui(-) phenotypes.
  • Mutations affecting translation initiation factors lead to derepressed Gcn4p translation (Gcd(-) phenotype).
  • Mutations impairing scanning or AUG recognition hinder Gcn4p induction (Gcn(-) phenotype).

Conclusions:

  • Phenotypic assays in yeast provide robust methods for dissecting translation mechanisms.
  • Specific mutations can be used to probe start codon selection and translational control.
  • These studies elucidate the complex regulation of protein synthesis, particularly for the Gcn4p activator.