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Updated: Jul 11, 2026

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
Published on: July 6, 2021
Real-time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods
Attilio Fabbretti1, Pohl Milon, Anna Maria Giuliodori
1Department of Biology MCA, University of Camerino, Camerino, Italy.
Abstract:
Three protocols to perform time-resolved in situ probing of rRNA are described. The three methods (chemical modification with DMS and rRNA backbone cleavage by hydroxyl radicals generated by either K-peroxonitrite or Fe(II)-EDTA) make use of a quench-flow apparatus and exploit reactions that are faster than the interactions of ribosomal subunits with their ligands. These methods allow the investigation of the path and dynamics, in a approximately equal 50 to 1500ms time range, of the binding and dissociation of ribosomal ligands.
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