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Published on: March 8, 2012
Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7
Zahra Meshkat1, Hoorieh Soleimanjahi, Mahmoud Mahmoudi
1Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Objective:
To isolate and construct a cloning vector containing the human papillomavirus (HPV)16-E7 gene as a target for application as a DNA vaccine.
Methods:
The study was performed in 2005 in Iran. The E7 gene, one of the most important HPV oncoproteins and a target molecule for therapeutic vaccines, was amplified by polymerase chain reaction (PCR). The PCR product was cloned into a suitable cloning vector and confirmed by colony-PCR, restriction enzyme analysis, and sequenced.
Results:
The desired plasmid was sequenced and indicated 99% homology with those mentioned in the Genbank.
Conclusion:
The Iranian HPV16 E7 gene sequence is very similar to other sequences in the Genbank, and it can be used as a candidate gene in a therapeutic vaccine for Iranian patients with cervical cancer.
