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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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Published on: February 16, 2018

Capacitive biosensor for detection of endotoxin.

Warakorn Limbut¹, Martin Hedström, Panote Thavarungkul

¹Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Box 124, 221 00, Lund, Sweden.

Analytical and Bioanalytical Chemistry

|October 5, 2007

Summary

A new capacitive biosensor detects bacterial endotoxin using horseshoe crab protein. This sensitive method offers a reliable and statistically validated approach for endotoxin quantification.

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Area of Science:

Biomedical Engineering
Biosensor Technology
Analytical Chemistry

Background:

Bacterial endotoxins are potent pyrogens requiring sensitive detection methods.
Current endotoxin detection methods, like the Limulus amebocyte lysate (LAL) test, can be time-consuming and costly.
Development of rapid, sensitive, and cost-effective biosensors is crucial for endotoxin monitoring.

Purpose of the Study:

To develop and validate a novel capacitive biosensor for the sensitive detection of bacterial endotoxin.
To immobilize endotoxin-neutralizing protein onto a gold transducer surface for enhanced detection.
To evaluate the performance of the capacitive biosensor against the conventional LAL test.

Main Methods:

Immobilization of endotoxin-neutralizing protein from American horseshoe crab onto a self-assembled thiol layer on a gold (Au) transducer.
Detection of endotoxin by measuring changes in the capacitive signal upon sample injection.
Quantitative analysis and comparison with the Limulus amebocyte lysate (LAL) test.

Main Results:

The capacitive biosensor demonstrated a low detection limit of 1.0 x 10(-13) M for endotoxin.
Linearity of detection ranged from 1.0 x 10(-13) M to 1.0 x 10(-10) M.
The biosensor results showed good agreement with the conventional LAL test, confirmed by statistical analysis (Wilcoxon signed rank test, P<0.05).

Conclusions:

The developed capacitive biosensor is a sensitive and reliable tool for bacterial endotoxin detection.
This biosensor offers a viable alternative to conventional methods for quantitative endotoxin analysis.
The system is statistically validated and acceptable for practical applications in endotoxin monitoring.