Alga-lysing bioreactor and dominant bacteria strain

Area of Science:

• Environmental Microbiology
• Water Treatment Technologies

Background:

• Alga-lysing bacteria are crucial for managing eutrophication.
• Strain P05, isolated from an immobilizing biosystem, shows promise for water remediation.

Purpose of the Study:

• To evaluate the efficacy of immobilized alga-lysing bacteria (strain P05) in biological reactors for treating eutrophic water.
• To compare the performance of coke and elastic filler as immobilization materials.

Main Methods:

• Immobilization of alga-lysing strain P05 on coke and elastic filler to create two biological reactors.
• Treatment of eutrophic water and assessment of removal efficiencies for algae (Chl-a), ammonia nitrogen (NH4(+)-N), and chemical oxygen demand (COD(Mn)).
• Identification of strain P05 using PCR amplification of 16S rRNA gene and BLAST analysis.

Main Results:

• Both reactors achieved stable and effective treatment after 7 days of biofilm formation.
• Optimal hydraulic retention time was determined to be 4 hours for both reactors.
• Algae removal rates (Chl-a) reached 80.38% (coke) and 82.1% (filler); NH4(+)-N removal was approximately 52.3-52.7%; COD(Mn) removal was around 39.03-39.64%.

Conclusions:

• Strain P05, identified as Bacillus sp., is an ideal alga-lysing bacterium for immobilization due to simple procedures and low-cost materials (coke, elastic filler).
• The developed biological reactors provide a stable, efficient, low-cost, and easy-to-operate method for eutrophic water treatment.