Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protease-sensitive fluorescent nanofibers.

Benedict Law1, Ralph Weissleder, Ching-Hsuan Tung

  • 1Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA. shek.law@ndsu.edu

Bioconjugate Chemistry
|October 6, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Intelligent catalase-coated MnO<sub>2</sub> nanoparticles with programmed oxygen supply and glutathione depletion for enhanced photodynamic therapy.

Journal of materials chemistry. B·2026
Same author

Tumor-Responsive Fluorogenic Spray for Image-Guided Cytoreduction of Disseminated Ovarian Tumors.

Bioconjugate chemistry·2025
Same author

Customized Nanofibers with Lung-Targeting and -Retention Properties for Treating Isolated Triple-Negative Breast Cancer Pulmonary Metastases.

ACS nano·2025
Same author

Iron-Doxorubicin Hyaluronan Nanogel as an Effective Targeted Chemotherapy with Modulated Cardiotoxicity.

Biomacromolecules·2025
Same author

Hijacking the hyaluronan assisted iron endocytosis to promote the ferroptosis in anticancer photodynamic therapy.

Carbohydrate polymers·2025
Same author

Editorial: Self-assembly of biomolecules as healthcare materials: drug delivery and beyond.

Frontiers in chemistry·2024
Same journal

Neutral Amphiphiles Boost Transfection Efficiency and Reduce Inflammation in Polymer Micelle-Mediated mRNA Delivery.

Bioconjugate chemistry·2026
Same journal

Surfactant-Mediated Buchwald-Hartwig Coupling of Aliphatic Amines for the Synthesis of DNA-Encoded Libraries.

Bioconjugate chemistry·2026
Same journal

Artificial Intelligence for Discovery in Life Sciences.

Bioconjugate chemistry·2026
Same journal

Iron Single Atom Nanozyme-Mediated GPX4 Inhibitor Delivery for Self-Enhanced Ferroptosis.

Bioconjugate chemistry·2026
Same journal

SpyCatcher-Engineered Ferritin Nanocages Enable Dual-Receptor Targeting for Enhanced Glioma Therapy.

Bioconjugate chemistry·2026
Same journal

One-Pot Synthesis of Functionalized Coumarin Fluorophores Enables Rapid Access to Live-Cell Bioorthogonal Labeling and Microenvironmental Sensing Agents.

Bioconjugate chemistry·2026
See all related articles

Researchers designed enzyme-responsive nanofibers using peptides and fluorescent reporters. These novel nanofibers release fluorescent fragments when exposed to specific enzymes like urokinase plasminogen activator (uPA), enabling potential diagnostic and drug delivery applications.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Enzyme-responsive materials are crucial for targeted therapies and diagnostics.
  • Self-assembled peptide nanofibers offer versatile platforms for biomedical applications.
  • Developing smart materials that respond to specific biological triggers is an active area of research.

Purpose of the Study:

  • To design and synthesize novel enzyme-responsive nanofibers.
  • To incorporate protease-sensitive domains and fluorescent reporters into peptide nanofibers.
  • To demonstrate the responsive release of fluorescent fragments triggered by a model enzyme.

Main Methods:

  • Self-assembly of hydrophobic beta-sheet peptides with PEG units.
  • Incorporation of protease-sensitive peptide sequences and fluorescent reporters.

Related Experiment Videos

  • Incubation with urokinase plasminogen activator (uPA) to trigger fiber degradation and fluorescence release.

    • Main Results:

    • Successful synthesis of enzyme-responsive nanofibers.
    • Demonstrated release of fluorescent fragments upon incubation with uPA.
    • Quantification of fluorescence release correlating with enzyme activity.

    Conclusions:

    • Enzyme-responsive nanofibers were successfully designed and synthesized.
    • The developed nanofibers exhibit controlled release of cargo in response to specific proteases.
    • These protease-sensitive nanofibers hold promise for biomedical applications such as diagnostic sensors and drug delivery systems.