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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

Embryonic stem cell differentiation studied by FT-IR spectroscopy.

Diletta Ami1, Tui Neri, Antonino Natalello

  • 1Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.

Biochimica Et Biophysica Acta
|October 6, 2007
PubMed
Summary

Fourier Transform Infrared (FT-IR) spectroscopy monitors embryonic stem (ES) cell differentiation by analyzing molecular changes. This rapid method tracks early cellular development and phenotype shifts at a molecular level.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Spectroscopy

Background:

  • Embryonic stem (ES) cells are crucial for developmental biology research.
  • Monitoring ES cell differentiation requires sensitive and rapid methods.

Purpose of the Study:

  • To develop and validate a Fourier Transform Infrared (FT-IR) spectroscopy method for monitoring spontaneous murine ES cell differentiation.
  • To identify key molecular changes indicative of early ES cell development.

Main Methods:

  • Utilized FT-IR spectroscopy to analyze ES cell samples at various differentiation stages.
  • Applied Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) for spectral data interpretation.
  • Correlated spectral data with cytochemical assays for validation.

Main Results:

  • Successfully segregated stem cell spectra based on differentiation time using PCA and LDA.
  • Identified significant spectral changes in protein amide I and nucleic acid regions (days 4-7).
  • Observed DNA/RNA hybrid bands, indicating early transcriptional genome switching.

Conclusions:

  • FT-IR spectroscopy provides a rapid, molecular-level insight into ES cell differentiation.
  • The method effectively tracks temporal evolution and phenotype changes during early development.
  • This approach offers a non-invasive way to monitor ES cell culture dynamics.