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Updated: Jul 11, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
Electrospray ionization and time-of-flight mass spectrometric method for simultaneous determination of spermidine and
Keijiro Samejima1, Masahiro Otani, Yasuko Murakami
1Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan. samejima@musashino-u.ac.jp
Abstract:
A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using (15)N-labeled polyamines.
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