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Related Concept Videos

SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

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The HNF-1alpha-SNARE connection.

K Yamagata1, T Nammo, Y Sato

  • 1Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. kazu@imed.med.osaka-u.ac.jp

Diabetes, Obesity & Metabolism
|October 9, 2007
PubMed
Summary

Maturity-onset diabetes of the young (MODY) is linked to HNF-1alpha gene mutations. Collectrin, a novel HNF-1alpha target, interacts with SNARE complexes to regulate insulin secretion.

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Genetics

Background:

  • Maturity-onset diabetes of the young (MODY) is a monogenic form of type 2 diabetes.
  • Hepatocyte nuclear factor (HNF)-1alpha gene mutations cause MODY type 3 (MODY3).
  • Pancreatic beta-cell dysfunction impairs glucose-stimulated insulin secretion in MODY.

Purpose of the Study:

  • To identify novel targets of HNF-1alpha in pancreatic beta-cells.
  • To investigate the role of collectrin in insulin secretion.
  • To elucidate the molecular mechanisms underlying MODY3.

Main Methods:

  • Investigated collectrin as a novel HNF-1alpha target gene.
  • Examined collectrin interaction with the SNARE complex via snapin.
  • Assessed collectrin's role in insulin exocytosis.

Main Results:

  • Collectrin is a novel HNF-1alpha target in pancreatic beta-cells.
  • Collectrin forms a complex with the SNARE complex through snapin.
  • Collectrin promotes SNARE complex formation and controls insulin exocytosis.

Conclusions:

  • Collectrin plays a crucial role in insulin secretion regulation.
  • Collectrin's interaction with the SNARE complex is vital for insulin exocytosis.
  • Collectrin represents a potential therapeutic target for MODY.