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Updated: Jul 11, 2026
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
Published on: July 30, 2011
Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants
Suvi Asikainen1, Markus Storvik, Merja Lakso
1Department of Neurobiology, A.I. Virtanen Institute, Kuopio University, Kuopio 70211, Finland.
Abstract:
We performed genome wide gene expression analysis on L4 stage Caenorhabditis elegans rrf-3(pk1426) and eri-1(mg366) mutant strains to study the effects caused by loss of their encoded proteins, which are required for the accumulation of endogenous secondary siRNAs. Mutant rrf-3 and eri-1 strains exhibited 72 transcripts that were co-over-expressed and 4 transcripts co-under-expressed (>2-fold, P<0.05) compared to N2 wild type strain. Ontology analysis indicated these transcripts were over represented for protein phosphorylation and sperm function genes. These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes.
