Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modelling the role of interaction heterogeneity in the gelation of micron-scale colloidal systems.

Soft matter·2026
Same author

Platinum-based therapeutics as emerging multi-modal radiosensitizers in glioblastoma treatment.

Advanced drug delivery reviews·2026
Same author

Data-Driven Feedback Identifies Focused Ultrasound Exposure Regimens for Improved Nanotheranostic Targeting of the Brain.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Slow relaxation and landscape-driven dynamics in viscous ripening foams.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Data-driven feedback augments ultrasound nanotheranostics in brain tumors.

bioRxiv : the preprint server for biology·2025
Same author

Fn14-Targeted Gold Nanorods for Augmenting Laser Thermal Therapy for High-Grade Gliomas.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: Jul 11, 2026

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
14:27

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity

Published on: August 19, 2013

Long-time stretched exponential kinetics in single DNA duplex dissociation.

Paul L Biancaniello1, Anthony J Kim, John C Crocker

  • 1Department of Physics and Astronomy, The University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Biophysical Journal
|October 9, 2007
PubMed
Summary

We studied DNA hybridization using single DNA molecules and optical tweezers. Our findings reveal complex dissociation pathways influencing DNA duplex stability.

More Related Videos

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

Related Experiment Videos

Last Updated: Jul 11, 2026

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
14:27

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity

Published on: August 19, 2013

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

Area of Science:

  • Biophysics
  • Molecular Biology
  • Genetics

Background:

  • Understanding DNA hybridization is crucial for molecular biology and genetics.
  • Previous studies on DNA duplex stability often involved significant molecular tension.
  • Investigating DNA kinetics under minimal force is essential for accurate modeling.

Purpose of the Study:

  • To investigate DNA hybridization kinetics at the single-molecule level.
  • To measure the lifetime distribution of single DNA duplexes during thermal dissociation.
  • To explore the role of molecular tension in DNA duplex stability.

Main Methods:

  • Utilizing an optical tweezer setup with two DNA-coated microspheres.
  • Measuring the dissociation lifetime of single 16-base pair (bp) DNA duplexes.
  • Conducting experiments under negligible molecular tension.

Main Results:

  • Observed a stretched exponential lifetime distribution for single DNA duplexes.
  • Identified dissociation proceeding via multiple competing pathways.
  • Found evidence of force-sensitive intermediate states influencing dissociation.
  • Noted unexpectedly short bound lifetimes for multiple DNA bridges, consistent with force sensitivity.

Conclusions:

  • DNA duplex dissociation is more complex than previously modeled, involving multiple pathways.
  • Force sensitivity plays a significant role in DNA duplex stability and dissociation kinetics.
  • The optical tweezer method provides a unique window into DNA behavior under minimal tension.