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Updated: Jul 11, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
Metabolic profiling using Fourier-transform ion-cyclotron-resonance mass spectrometry
Daisaku Ohta1, Daisuke Shibata, Shigehiko Kanaya
1Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Japan. ohtad@bioinfo.osakafu-u.ac.jp
Abstract:
With the aid of the extreme resolving power of Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR/MS), we have developed a metabolomics platform for high-throughput metabolic profiling and metabolite candidate identification integrating a data-processing system, the Dr.DMASS program ( http://kanaya.naist.jp/DrDMASS/ ), and a metabolite-species database, KNApSAcK ( http://kanaya.aist-nara.ac.jp/KNApSAcK/ ). We discuss the potential of this FT-ICR/MS-based metabolic profiling scheme as a general metabolomics tool by clarification of plant metabolic disorders and specific metabolite accumulation patterns caused by herbicidal enzyme inhibitors.
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