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Updated: Jul 11, 2026
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
[Identification of a novel HLA allele B 4446 by sequencing-based typing]
Jian-ping Li1, Xu Zhang, Xiao-feng Li
1Liaoning Blood Center, Shenyang, Liaoning, 110044 P. R. China. 123@syxz.com.
Objective:
To identify a novel HLA allele in Chinese population.
Methods:
A new HLA-B allele was initially detected by an usual PCR-SSP and PCR-SSOP in routine typing HLA allele. Sequence-based typing (SBT) was used to identify and analysis the difference between the new allele and HLA-B 4409 allele.
Results:
The HLA-B exon 3 nucleotide sequence of the novel allele was different from all other known alleles. The allele had 3 nucleotides replaced of the closest matching B 4409 allele at nt538(G>C), nt539(A>T) and nt540 (C>G) in exon 3, resulting in an amino acid change from D(GAC) to L(CTG) at codon 180.
Conclusion:
A novel HLA allele was confirmed by the SBT and it was officially designated as HLA-B 4446 by WHO Nomenclature Committee for Factors of the HLA System in September,2005.
