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Updated: Jul 11, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Published on: April 1, 2016
Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries
Tuck Seng Wong1, Danilo Roccatano, Ulrich Schwaneberg
1Jacobs University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany.
Abstract:
How to explore protein sequence space efficiently and how to generate high-quality mutant libraries that allow to identify improved variants with current screening technologies are key questions for any directed protein evolution experiment. High-quality mutant libraries can be generated through improved random mutagenesis methodologies and by restricting diversity generation through computational methods to residues which have high success probabilities. Advances in mutant library design and computational tools to focus diversity generation are summarized in this minireview and discussed from an experimentalist point of view in the context of directed protein evolution.
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