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Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Related Experiment Video

Updated: Jul 11, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Single-cell genomic sequencing using Multiple Displacement Amplification.

Roger S Lasken¹

¹J. Craig Venter Institute, 11149 N. Torrey Pines Rd., Suite 200, La Jolla, CA 92037, USA. rlasken@jcvi.org

Current Opinion in Microbiology

|October 10, 2007

Summary

Multiple Displacement Amplification (MDA) enables sequencing of single microbial cells. This DNA amplification technique accelerates the study of uncultured microbes and aids in assembling metagenomic sequences.

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Last Updated: Jul 11, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Published on: May 23, 2018

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Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Published on: May 15, 2018

Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Sequencing microbial genomes is crucial for understanding microbial life.
Culturing microbes from environmental samples remains a significant challenge.
Existing methods for single-cell DNA analysis are limited.

Purpose of the Study:

To evaluate Multiple Displacement Amplification (MDA) for single microbial cell DNA sequencing.
To assess the suitability of MDA-amplified DNA for various sequencing technologies.
To explore the utility of single-cell MDA in metagenomics.

Main Methods:

DNA amplification from single bacterial cells using Multiple Displacement Amplification (MDA).
Construction of DNA libraries from amplified DNA for Sanger sequencing.
Direct use of MDA-generated DNA as a template for 454 pyrosequencing.

Main Results:

MDA successfully amplified minute amounts of DNA from single cells into micrograms of high molecular weight DNA.
MDA-generated DNA was suitable for both Sanger sequencing library construction and 454 pyrosequencing.
While some genomic sequence was lost, MDA significantly accelerated sequencing of uncultured microbes.

Conclusions:

Multiple Displacement Amplification (MDA) is a powerful tool for single microbial cell sequencing.
This technique facilitates the genomic analysis of previously uncultured microorganisms.
MDA-derived single-cell sequences aid in guiding the assembly of complex metagenomic data.