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[The construction of ADAM28 eukaryotic expression plasmid and expression after transfected into HDFC]
Zheng Zhao1, Zheng-nan Huang, Jun-ming Xu
1Department of Stomatology, The 401 Hospital of PLA, Qingdao 266071, China. zhaozheng170@sohu.com
Purpose:
To construct a disintegrin and metalloproteinase (ADAM28) eukaryotic expression plasmid and detect the expression of ADAM28 gene in human dental follicle cells (HDFC) after eukaryotic plasmid was transfected into HDFC.
Methods:
Gene rebuilt technique was used to construct ADAM28 eukaryotic expression plasmid, cell culture and gene transfection into HDFC mediated by Lipofectamine2000, immunofluorescence, RT-PCR and Western blot were used to detect the expression of ADAM28 in HDFC.
Results:
ADAM28 eukaryotic plasmid was constructed, identified successfully and transiently transfected into HDFC for 72 hours. The detection verified that HDFC of eukaryotic plasmid group all expressed ADAM28 protein, and no expression was found in two control groups.
Conclusions:
ADAM28 could be correctly translated and expressed in HDFC, which might be used for further study of biological functions of ADAM28 in odontogenic cells.
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