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CD36 polymorphism and its relationship with body mass index and coronary artery disease in a Korean population
Yeo Min Yun1, Eun Young Song, Sang Hoon Song
1Department of Laboratory Medicine, Konkuk University College of Medicine, Seoul, Korea.
Insights
Genetic variations in the CD36 gene are linked to increased body mass index (BMI) and cardiovascular disease risk in Korean men. Specifically, (TG) repeat polymorphisms in intron 3 are associated with higher BMI and coronary artery disease (CAD) risk.
Area of Science:
- Genetics
- Cardiovascular Medicine
- Molecular Biology
Background:
- CD36 is a key membrane receptor and cell-adhesion molecule found in various cell types, including platelets and endothelial cells.
- Genetic variations in CD36 may influence susceptibility to diseases like coronary artery disease (CAD).
Purpose of the Study:
- To investigate the association between CD36 gene polymorphisms and the risk of coronary artery disease (CAD) in a Korean population.
- To explore the relationship between specific CD36 genetic variations and cardiovascular risk factors such as body mass index (BMI).
Main Methods:
- Genotyping of CD36 gene polymorphisms, including a (TG) repeat in intron 3 and a 478C>T base substitution, using PCR and RFLP.
- Analysis of genotypes, lipid profiles, and other CAD risk factors in 219 CAD patients and 236 control subjects.
Main Results:
- A significantly higher frequency of (TG) 11- or 12-repeat homozygotes was observed in male CAD patients compared to control men (28.4% vs. 15.7%).
- Homozygosity for the (TG) 11- or 12-repeat allele was associated with higher BMI in control men, explaining 13% of BMI variation.
- No significant difference in the 478C>T allele frequency was found between CAD patients and controls, but this allele was linked to the (TG) 11- or 12-repeat allele in controls.
Conclusions:
- The (TG) repeat polymorphism in intron 3 of the CD36 gene is associated with increased BMI and cardiovascular risk in men.
- These findings suggest a potential genetic predisposition to CAD in the Korean population related to CD36 gene variations.
Background:
CD36 is a multifunctional membrane receptor and a cell-adhesion molecule that is expressed in platelets, monocytes/macrophages, microvascular endothelial cells, cardiac monocytes and adipocytes. In this study, we investigated whether genetic polymorphisms of the CD36 gene are associated with risk of coronary artery disease (CAD) in a Korean population.
Methods:
PCR and polyacrylamide gel electrophoresis or PCR-restriction fragment length polymorphism assays were performed to analyze the following CD36 gene polymorphisms: a (TG) repeat in intron 3 and the base substitution 478C>T (Pro90Ser). A total of 219 patients with significant CAD and 236 control subjects were examined with regard to their genotypes, lipid profiles and other risk factors for CAD.
Results:
The frequency of (TG) 11- or 12-repeat homozygotes was significantly higher in male CAD patients than in control men (28.4% vs. 15.7%, OR=2.13, p=0.018). Homozygosity for the (TG) 11- or 12-repeat allele was also significantly associated with a higher body mass index (BMI) compared to non-carriers in 134 control men after controlling for age, smoking and hypertension, and explains a 13% BMI variation observed in this study (p=0.015, analysis of covariance). For the 478C >T mutation, which has been reported to be associated with CD36 deficiency, there was no difference in the frequency of the 478T allele between CAD patients and control subjects. However, the 478T allele was found to be closely linked with a (TG) 11- or 12-repeat allele of intron 3 in the control subjects (chi2=18.88, p<0.001).
Conclusions:
The (TG) repeat polymorphism in intron 3 of the CD36 gene is associated with a higher BMI and cardiovascular risk for men in a Korean population.
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