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Cytokines and growth factors in mostly atherosclerotic patients on hemodialysis determined by biochip array
Jadranka Sertic1, Jasna Slavicek, Nada Bozina
1Department of Clinical Chemistry, Zagreb University School of Medicine, Zagreb, Croatia. jadranka.sertic@kbc-zagreb.hr
Insights
This study found significant differences in cytokine and growth factor levels between chronic renal failure (CRF) patients on hemodialysis (HD) and healthy controls. These markers may help diagnose and predict outcomes in CRF patients.
Area of Science:
- Nephrology
- Immunology
- Cardiovascular Research
Background:
- Chronic renal failure (CRF) reduces lifespan, with coronary artery disease as a primary cause of mortality.
- Atherosclerosis progression and impaired angiogenesis are characteristic of CRF.
- Inflammation markers and growth factors are crucial for understanding vascular pathology in CRF.
Purpose of the Study:
- To quantify plasma levels of various cytokines and growth factors in hemodialysis (HD) patients.
- To compare these levels with those in a control group.
- To identify potential diagnostic and prognostic markers for CRF.
Main Methods:
- Plasma concentrations of cytokines (IL-2, IL-4, IL-6, IL-8, IL-10, IL-1 alpha, IL-1 beta) and growth factors (VEGF, EGF, IFN gamma, TNFalpha, MCP-1) were measured.
- An Evidence((R)) biochip array analyzer was utilized for quantification.
- HD patients (n=75) were compared to control subjects (n=113).
Main Results:
- HD patients exhibited higher levels of IL-6, IL-8, IL-10, TNFalpha, IL-1 beta, and MCP-1 compared to controls.
- Conversely, IL-2, IL-4, and EGF levels were higher in controls.
- Specific cytokine levels varied significantly based on gender, age, duration of HD, underlying renal disease, and presence of atherosclerosis or HCV infection.
Conclusions:
- Plasma levels of certain cytokines and growth factors show significant differences in CRF patients undergoing HD.
- These measured biomarkers hold potential as valuable diagnostic and prognostic tools for CRF patients.
- Further research can elucidate the precise role of these markers in CRF vascular complications.
Background:
The lifespan of patients with chronic renal failure (CRF) is reduced, and coronary artery disease is the leading cause of morbidity and mortality in these patients. The progression of atherosclerosis is accelerated and angiogenesis is impaired in CRF. Risk factors that could contribute to further understanding of vascular pathology include markers of inflammation and growth factors. The purpose of this study was to determine the levels of cytokines (IL-2, IL4, IL-6, IL-8, IL-10, IL-1 alpha, IL-1 beta), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), interferon-gamma (IFN gamma), tumor necrosis factor-alpha (TNFalpha) and monocyte chemotactic protein-1 (MCP-1) in patients on chronic hemodialysis (HD; n=75), and to compare values with those of control subjects (n=113).
Methods:
Evidence((R)) biochip array analyzer was used for quantification of plasma concentrations in samples.
Results:
Significant differences were found between the control subjects and HD patients. IL-2 (p<0.001), IL-4 (p<0.001) and EGF (p<0.001) levels were higher in controls than in HD patients, while IL-6 (p<0.001), IL-8 (p=0.081), IL-10 (p=0.008), TNFalpha (p<0.001), IL-1 beta (p<0.001) and MCP-1 (p<0.001) levels were higher in HD patients. We also found IL-2 (p=0.015) and IL-1 alpha (p=0.035) levels to be significantly higher in males than females, while IL-4 (p=0.025) and IL-1 beta (p=0.049) levels were significantly higher in females. Among HD patients, IL-2 levels were higher in patients under the age of 50 years (p<0.048). It was also higher in female than in male patients (p<0.035) and in patients on HD for more than 10 years (p<0.009). IL-6 levels were higher in patients over the age of 50 years (p<0.047). Patients with previous glomerulonephritis had the highest level of IL-6 compared to patients with previous pyelonephritis and diabetes mellitus (p<0.063). IL-6 levels were higher in patients with concomitant hepatitis C virus (HCV) infection (p<0.036) and in patients with developed atherosclerosis (p<0.003). IL-8 levels were higher in patients over the age of 50 years (p<0.003) and in the group with previous glomerulonephritis (p<0.031). IL-10 levels were higher in the group with developed atherosclerosis (p<0.045). EGF was the highest in the group of patients with previous diabetes mellitus compared to pyelonephritis and glomerulonephritis groups (p<0.073). TNFalpha levels were higher in the patient population on HD for more than 10 years (p<0.032) and in the concomitant HCV group (p<0.073). IL-1 beta levels were higher in the HCV group (p<0.088).
Conclusions:
Plasma concentrations of some cytokines and growth factors could serve as useful diagnostic and prognostic parameters for patients with CRF on HD.
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