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Related Experiment Video

Updated: Jul 11, 2026

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods

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An EST database from saffron stigmas.

Nunzio D'Agostino¹, Daniele Pizzichini, Maria Luisa Chiusano

¹ENEA, Casaccia Research Center, PO Box 2400, Roma 00100AD, Italy. nunzio.dagostino@gmail.com

BMC Plant Biology

|October 11, 2007

Summary

Researchers created a searchable database of saffron stigma genes, identifying key genes for its unique color and flavor. This resource aids understanding of saffron

Area of Science:

Plant genomics
Molecular biology
Biochemistry

Background:

Saffron (Crocus sativus L.) has historical medicinal and culinary uses.
Saffron stigmas are the source of its valuable color and flavor compounds.
Stigmas exhibit unique secondary metabolism crucial for saffron's properties.

Purpose of the Study:

To generate and characterize expressed sequence tags (ESTs) from saffron stigmas.
To establish a searchable database for saffron stigma gene discovery.
To investigate the molecular basis of saffron's secondary metabolism.

Main Methods:

Construction of a saffron stigma cDNA library.
Generation and quality assessment of 6,603 high-quality Expressed Sequence Tags (ESTs).

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Clustering and annotation of ESTs to identify unique genes.

Main Results:

1,893 unique expressed genes (Clusters) were identified from the ESTs.
The Saffron Genes database (http://www.saffrongenes.org) was created for data access.
Identified sequences include genes for sex determination, lipid/carotenoid metabolism, and transcription factors.

Conclusions:

The Saffron Genes database is a foundational resource for Iridaceae genomics.
Provides insights into stigma development and saffron's secondary metabolism.
Facilitates further research into saffron's molecular biology and metabolic pathways.