Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantum dot imaging for embryonic stem cells.

Shuan Lin1, Xiaoyan Xie, Manishkumar R Patel

  • 1Molecular Imaging Program at Stanford (MIPS) and Bio-X Program, Department of Radiology, Stanford University, Stanford, CA 94305, USA. susans1018@gmail.com

BMC Biotechnology
|October 11, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness of Home Care Interventions in Patients with Moderate to Severe Craniocerebral Injury Combined with Epilepsy.

Computational and mathematical methods in medicine·2022
Same author

Plumbagin protects H9c2 cardiomyocytes against TBHP-induced cytotoxicity by alleviating ROS-induced apoptosis and modulating autophagy.

Experimental and therapeutic medicine·2022
Same author

Extracellular Vesicle/Macrophage Axis: Potential Targets for Inflammatory Disease Intervention.

Frontiers in immunology·2022
Same author

Astrocyte reactivation in medial prefrontal cortex contributes to obesity-promoted depressive-like behaviors.

Journal of neuroinflammation·2022
Same author

Primary sarcomatoid carcinoma of the ileum: Genetic alterations and treatment.

Asian journal of surgery·2022
Same author

Identification of key microRNAs in exosomes derived from patients with the severe acute pancreatitis.

Asian journal of surgery·2022
Same journal

Editorial Expression of Concern: Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus.

BMC biotechnology·2026
Same journal

Computer-guided enzyme engineering of PET hydrolase mutants towards improved PET affinity.

BMC biotechnology·2026
Same journal

Synthesis and application of exopolysaccharide-selenium nanocomposite produced by Pseudoduganella armeniaca ZMN-3.

BMC biotechnology·2026
Same journal

Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data.

BMC biotechnology·2026
Same journal

Comparative analysis of MinION and MiSeq using 16S rRNA gene amplicon sequencing in human gut microbiome.

BMC biotechnology·2026
Same journal

Integrated structure-based and systems-level identification of PDE9A inhibitors for heart failure with preserved ejection fraction.

BMC biotechnology·2026
See all related articles

Semiconductor quantum dots (QDs) enable in vivo multiplex imaging of mouse embryonic stem cells without affecting cell viability. QD 800 demonstrated superior fluorescent intensity for tracking stem cells in vivo.

Area of Science:

  • Biomedical imaging
  • Nanotechnology
  • Stem cell biology

Background:

  • Semiconductor quantum dots (QDs) are increasingly utilized for in vitro and in vivo cellular imaging.
  • This study evaluates the efficacy of Qtracker-delivered QDs for in vivo multiplex imaging of mouse embryonic stem (ES) cells.

Purpose of the Study:

  • To assess the feasibility of in vivo multiplex imaging of mouse ES cells labeled with QDs.
  • To determine the impact of QD labeling on ES cell viability, proliferation, and differentiation.
  • To compare the fluorescent intensity of different QDs for in vivo imaging.

Main Methods:

  • Mouse ES cells were labeled with six distinct QDs using Qtracker.
  • Cell viability, proliferation, and differentiation assays were performed.

Related Experiment Videos

  • Labeled ES cells were injected subcutaneously into athymic nude mice for in vivo imaging.
  • Fluorescent signal intensity was measured using a single excitation wavelength.

    • Main Results:

    • QD labeling did not adversely affect ES cell viability, proliferation, or differentiation compared to controls.
    • In vivo imaging of labeled ES cells was achieved with good contrast using a single excitation wavelength.
    • QD 800 exhibited significantly higher fluorescent intensity (1,713 +/- 482) compared to other tested QDs (QD 525-705).

    Conclusions:

    • This study demonstrates the first in vivo multiplex imaging of QD-labeled mouse ES cells.
    • QDs show promise as a tool for non-invasive in vivo imaging of stem cell therapy.
    • Further advancements in QD technology could enhance stem cell tracking within deep tissues.