[The in vitro testing of the sensitivity of human cryopreserved pulp cells]

The purpose of this in vitro study was to evaluate the sensibility of frozen human pulp cells, in order to examine the cytotoxicity of dental products. The cell cultures and the biological assessments, using still incomplete human premolars and pulp germs, were achieved according to a standardized method. The sensibility of frozen cells was assessed by comparison of cell reactions before and after 1, 3 and 6 months of congelation, for two dental products called Aquacem and Pulpdent, the cytotoxicity and biocompatibility of which were further confirmed. The behaviour of cells with and without congelation was microscopically evaluated, the growth and morphology of cells were assessed after 7, 14, 21 and 28 days. The comparison of the results showed a similar behaviour between cells with and without congelation, except for few cell alterations in the form of granula which occurred during a short period, probably due to the congelation process. This cell liability offers the opportunity to elaborate a bank of cells available on demand for preliminary cytotoxicity assessments.