Related Experiment Video
Updated: Jul 11, 2026
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
Published on: January 29, 2022
Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein
C C Berry1, A S G Curtis, R O C Oreffo
1Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK. Catherine.berry@bio.gla.ac.uk
Abstract:
The separate influence of topographical and chemical cues on cell attachment and spreading are well documented; however, that of duel-cue substrates is less so. In this study graft copolymers that sterically stabilize biological surfaces were employed alongside nanotopographical features fabricated by colloidal lithography. This resulted in the production of a range of substrates whereby the effect of chemistry and or topography on both on human fibroblast and bone marrow cell adhesion and spreading could be observed. The current studies indicate an enhancement of cell response as a consequence of modifications in material topography, whereas the current selected chemical cues inhibited cell function. Critically, in combination, topography modulated the effects of chemical environment.
