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Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology
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High-throughput immunophenotyping by surface plasmon resonance imaging.

Koichi Kato1, Toshinari Ishimuro, Yusuke Arima

  • 1Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

Analytical Chemistry
|October 12, 2007
PubMed
Summary

This study introduces surface plasmon resonance (SPR) imaging for quantitative cell binding detection on antibody arrays. This breakthrough enhances immunophenotyping throughput by enabling parallel and precise analysis of captured cells.

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Area of Science:

  • Biotechnology
  • Immunology
  • Analytical Chemistry

Background:

  • Cell binding assays on antibody arrays enable rapid immunophenotyping.
  • Current methods face throughput limitations due to challenges in parallel and quantitative cell detection.

Purpose of the Study:

  • To develop and validate an imaging technique for quantitative cell detection on antibody arrays.
  • To overcome the throughput limitations of current cell-based immunophenotyping methods.

Main Methods:

  • Fabrication of antibody arrays via photopatterning of alkanethiol monolayers on gold-coated glass.
  • Immobilization of antibodies onto distinct spots for parallel binding assays.
  • Utilizing surface plasmon resonance (SPR) imaging to monitor refractive index changes caused by cell binding.
  • Employing confocal laser scanning microscopy to analyze cell-antibody interactions.

Main Results:

  • SPR imaging successfully detected intensified signals on specific antibody spots corresponding to cell capture.
  • Observed SPR signals correlated with cell deformations resulting from multivalent antibody interactions.
  • Quantitative monitoring of cell binding to multiple antibody spots from heterogeneous cell populations was achieved.

Conclusions:

  • SPR imaging offers a novel, quantitative method for analyzing cell binding on antibody arrays.
  • This technique significantly enhances the throughput and precision of immunophenotyping.
  • The method holds potential for applications in diagnostics and drug discovery involving cell-surface interactions.