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Updated: Jul 11, 2026
Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
Published on: January 15, 2014
Pattern formation of antifreeze glycoproteins via solvent evaporation
Osnat Younes-Metzler1, Robert N Ben, Javier B Giorgi
1Center for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie Street, Ottawa, Ontario, Canada K1N 6N5.
Abstract:
Surface patterning of antifreeze glycoprotein fraction 8 (AFGP 8) via a solvent evaporation method is reported here. In this process, lines of AFGP 8 particles and gridlike patterns were formed as as result of the receding of the droplet contact line and the accumulation of the solute during evaporation. The solution concentration strongly affects the protein line spacing. The average height of the protein was measured to be 8.1 +/- 2.5 A, which may be attributed to the height of a single molecule.
