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Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells

Tomoko Nakanishi1, Satoshi Kumagai, Masanori Kimura

  • 1Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. tomo@grape.med.tottori-u.ac.jp

Biochemical and Biophysical Research Communications
|October 12, 2007
PubMed

Insights

Mouse GLD-2 (mGLD-2) is not essential for oogenesis or development. Disrupted mice show normal poly(A) tail elongation, indicating alternative cytoplasmic polyadenylation pathways exist in mice.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Cytoplasmic polyadenylation is crucial for oogenesis and early embryogenesis.
  • mGLD-2 is the mouse homolog of Xenopus cytoplasmic poly(A) polymerase xGLD-2.
  • mGLD-2 associates with known polyadenylation factors CPEB and CPSF.

Purpose of the Study:

  • To investigate the role of mGLD-2 in mouse oogenesis and development.
  • To determine if mGLD-2 is essential for cytoplasmic poly(A) tail elongation in mouse oocytes.

Main Methods:

  • Generated mGLD-2 disrupted mouse line using homologous recombination.
  • Assessed poly(A) tail elongation in oocytes using reporter RNAs.
  • Evaluated oocyte maturation and mouse fertility.

Main Results:

  • mGLD-2 is ubiquitously expressed but not essential for mouse development or fertility.
  • mGLD-2 disruption did not impair poly(A) tail elongation in oocytes.
  • Oocyte maturation and fertility were normal in disrupted mice.

Conclusions:

  • mGLD-2 is dispensable for full-term development and oogenesis in mice.
  • The results suggest the presence of alternative sources for cytoplasmic poly(A) polymerase activity in mice.

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