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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
Published on: January 7, 2019
Multiwall carbon nanotube scaffolds for tissue engineering purposes
Ander Abarrategi1, María C Gutiérrez, Carolina Moreno-Vicente
1Instituto de Estudios Biofuncionales, Universidad Complutense, Paseo Juan XXIII 1, Madrid, Spain.
Biomaterials
|October 12, 2007
Summary
This study explores multiwall carbon nanotube/chitosan scaffolds for cell culture. These biocompatible scaffolds support C2C12 cell growth and osteoblastic differentiation, showing potential for bone tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Biocompatible and biodegradable scaffolds are crucial for tissue regeneration.
- Multiwall carbon nanotubes (MWCNT) offer unique properties but require integration with biocompatible polymers.
- Chitosan (CHI) is a well-established biomaterial for tissue engineering applications.
Purpose of the Study:
- To evaluate multiwall carbon nanotube/chitosan (MWCNT/CHI) composite scaffolds as supports for cell culture.
- To assess the biocompatibility, cell adhesion, viability, and proliferation of C2C12 cells on MWCNT/CHI scaffolds.
- To investigate the potential of MWCNT/CHI scaffolds to promote osteoblastic differentiation of C2C12 cells in the presence of recombinant human bone morphogenetic protein-2 (rhBMP-2).
Main Methods:
- Fabrication of MWCNT/CHI scaffolds with a microchannel porous structure (up to 89 wt.% MWCNT).
- In vitro evaluation of C2C12 cell adhesion, viability, and proliferation using MTT assays.
- In vitro assessment of osteoblastic differentiation via alkaline phosphatase activity.
- In vivo study involving implantation of rhBMP-2-adsorbed scaffolds in muscle tissue to evaluate ectopic bone formation.
Main Results:
- MWCNT/CHI scaffolds demonstrated good biocompatibility, supporting C2C12 cell adhesion, viability, and proliferation.
- In vitro studies showed that rhBMP-2 adsorbed onto MWCNT/CHI scaffolds induced osteoblastic differentiation of C2C12 cells.
- In vivo implantation resulted in the ectopic formation of bone tissue, confirming the osteoinductive potential of the scaffolds.
Conclusions:
- MWCNT/CHI composite scaffolds are promising biocompatible and biodegradable materials for cell culture and tissue engineering.
- These scaffolds effectively support cell growth and can promote osteoblastic differentiation and bone formation when combined with rhBMP-2.
- The findings suggest potential applications in regenerative medicine, particularly for bone defect repair.
