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Updated: Jul 11, 2026

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
Published on: January 31, 2019
ATP plays a role in neurite stimulation with activated mast cells
Ryo Suzuki1, Tadahide Furuno, Keisuke Okamoto
1Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.
Abstract:
Previously, we showed that nerve-mast cell cross-talk can occur bidirectionally and that substance P is a mediator to activate mast cells. Here, we have studied the mediators to activate nerves cocultured with mast cells. Addition of antigen to the cocultures of superior cervical ganglia (SCG) and rat basophilic leukemia cells (RBLs) elicited Ca(2+) response in RBLs and after a lag period induced Ca(2+) signal in SCG neurites. Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (purinergic receptor antagonist) or apyrase (ATP-hydrolyzing enzyme) reduced the Ca(2+) signals in neurites, indicating that ATP released from activated mast cells was one of important mediators to activate nerves.
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