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Evaluation of behavioral differences between native polycyclic aromatic hydrocarbons and (13)C-labeled internal

Nobuyasu Itoh1, Masahiko Numata, Takashi Yarita

  • 1National Metrology Institute of Japan (NMIJ), Japan. nobuyasu-itoh@aist.go.jp

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|October 12, 2007
PubMed

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Chemical Shift: Internal References and Solvent Effects01:17

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In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...