[Facilitation of Shengjingbao to spermatogenesis in oligospermia mouse models and its action mechanisms]
Related Experiment Video
Updated: Jul 11, 2026
Mouse Round Spermatid Injection
Published on: January 26, 2024
Li Yang1, Ji-hong Shen, Jin-kun Li
1Department of Urology, the First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China.
Objective:
To investigate the effects of Shengjingbao on spermatogenesis in the mouse model of oligospermia and its action mechanisms.
Methods:
Sixty male mice were randomly divided into 4 groups, Shengjingbao (Group 1), Vitamin E (Group 2), blank model control (Group 3) and normal blank control (Group 4). The first three groups were treated by celiac injection of cyclophosphamide for 5 successive days to make models, followed by intragastric administration of Shengjingbao and Vitamin E to Group I and 2, respectively, for 36 days. And then all the mice were sacrificed. The serum testosterone (T) level was determined by radioimmunology, and a suspension was made from the testis and epididymis of one side for sperm analysis, while the testis of the other side was sliced up and stained by HE method and TUNEL technique to detect the count of Leydig cells, the layers of spermatogenic epithelia and the apoptosis of spermatogenic cells.
Results:
Compared with Group 3, the serum T level, the layers of spermatogenic epithelia and the count of Leydig cells were obviously improved and even exceeded those in Group 2. The positive expression rate of spermatogenic cell apoptosis in Group 1 was evidently lower than Group 2 and 3. The above differences were statistically significant (P < 0.01 or P < 0.05), but no significant difference was noted between Group 1 and 4.
Conclusion:
Shengjingbao can significantly increase the count of Leydig cells, elevate the T level that influences the layers of spermatogenic epithelia, and thus enhance spermatogenesis. The action mechanisms of Shengjingbao may lie in its capacity of inhibiting the apoptosis of spermatogenic cells, increasing the layers of spermatogenic epithelia and facilitating spermatogenesis. T may play a role in inhibiting the apoptosis of spermatogenic cells.
Insights
Shengjingbao significantly improved sperm production in mice with low sperm count. It increased Leydig cells, testosterone levels, and spermatogenic epithelia layers while reducing sperm cell apoptosis.
Area of Science:
- Reproductive biology
- Pharmacology
- Andrology
Context:
- Oligospermia, characterized by low sperm count, is a significant cause of male infertility.
- Understanding the mechanisms behind male infertility is crucial for developing effective treatments.
Purpose:
- To evaluate the efficacy of Shengjingbao in improving spermatogenesis in a mouse model of oligospermia.
- To elucidate the underlying mechanisms of Shengjingbao's action on sperm production.
Summary:
- Shengjingbao treatment in oligospermic mice led to significant increases in serum testosterone (T) levels, Leydig cell counts, and spermatogenic epithelia layers compared to controls.
- The herbal formula notably reduced the apoptosis rate of spermatogenic cells, suggesting a protective effect.
- These improvements in spermatogenesis were comparable to or exceeded those observed with Vitamin E treatment.
Impact:
- Shengjingbao demonstrates potential as a therapeutic agent for male infertility associated with oligospermia.
- The findings suggest that Shengjingbao enhances spermatogenesis by modulating Leydig cell function, T levels, and inhibiting germ cell apoptosis.
- Further research into Shengjingbao could lead to novel treatments for improving male reproductive health.
