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Direct measurements of the stabilization of single-stranded DNA under tension by single-stranded binding proteins

K Hatch1, C Danilowicz, V Coljee

  • 1Department of Physics, Harvard University, Cambridge, Massachusetts 02138, USA.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|October 13, 2007
PubMed

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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...