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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Cell-based high-content screening of small-molecule libraries.

Kerstin Korn¹, Eberhard Krausz

¹HT-Technology Development Studio (TDS), Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Pfotenhauerstrasse 108, D-01307 Dresden, Germany.

Current Opinion in Chemical Biology

|October 13, 2007

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Cell-based high-content screening (HCS) uses advanced microscopy to analyze cellular responses to chemical or genetic changes. This powerful method aids in discovering new therapeutic compounds by examining cellular networks.

Area of Science:

Cell Biology
Biotechnology
Pharmacology

Background:

Advanced microscopy and image analysis are crucial for studying cellular events.
Cell-based high-content screening (HCS) is an emerging technique for investigating cellular processes and alterations.
HCS enables multiparametric characterization of cellular responses using live cells as reporters.

Purpose of the Study:

To highlight the capabilities of cell-based high-content screening (HCS) in drug discovery.
To emphasize the advantages of HCS over traditional biochemical screening methods.
To showcase the application of HCS in identifying and characterizing novel therapeutic lead compounds.

Main Methods:

Utilizing advanced microscopy and image analysis for cellular event studies.

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Employing cell-based high-content screening (HCS) for investigating cellular processes.

Performing multiparametric characterization of cellular responses to chemical or genetic perturbations.

Screening for effects on molecular and cellular targets, including protein localization and redistribution.

Main Results:

HCS allows for the analysis of cellular responses within the context of intercellular networks.
It provides a more holistic view compared to biochemical screening methods.
Large-scale image-based screens with over 100,000 compounds have been demonstrated.

Conclusions:

Cell-based HCS is a powerful and emerging methodology for drug discovery.
HCS offers a comprehensive approach to understanding cellular behavior and responses.
The technology is actively driven by pharmaceutical companies for identifying therapeutic leads.