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Updated: Jul 10, 2026

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
Published on: January 30, 2020
Corrections to facilitate planar imaging of particle concentration in particle-laden flows using Mie scattering. Part
Peter A M Kalt1, Graham J Nathan
1School of Mechanical Engineering, University of Adelaide, Adelaide SA 5005, Australia. pkalt@mecheng.adelaide.edu.au
Abstract:
Part 1 describes a model to account for the effect of particles on laser sheet attenuation in flows where particles are heterogeneously distributed and where particles are small compared with the imaged volume. Here we extend the model to account for the effect of a strongly diverging light sheet, which is desirable when investigating many turbulent flows, e.g., in two-phase combustion problems. A calibration constant, C(kappa), is derived to account for the attenuation of the incident laser sheet due to extinction of the laser beam through a seeded medium. This is shown to be effective in correcting both the effect of in-plane laser sheet attenuation and out-of-plane signal trapping due to particles in a jet flow heavily seeded with 5 g/s of 25-40 microm spherical particles. In the uncorrected case, attenuation causes up to 15% error in the mean concentration and 35% error on the rms fluctuations. Selecting an appropriate C(kappa) was found to remove the error in the mean concentration and reduce error on the rms fluctuation by half. Methods to estimate or measure an appropriate value of C(kappa) are also presented.

