Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons.

Nature communications·2026

Same author

Evolutionary dynamics of Respiratory Syncytial Virus in pre-pandemic, pandemic, and post-pandemic periods in Houston, Texas, USA.

bioRxiv : the preprint server for biology·2026

Same author

Publisher Correction: Whole genome sequence analysis of pulmonary function and COPD in 44,287 multi-ancestry participants.

Genome biology·2026

Same author

<i>PTPN1</i>-related autoinflammation is a common cause of Aicardi-Goutières Syndrome with reduced penetrance.

medRxiv : the preprint server for health sciences·2026

Same author

Environmental Drivers of Genetic Structure and Local Adaptation in a Marine Foundation Species.

Ecology and evolution·2026

Same author

Overcoming host restrictions to enable continuous passaging of GII.3 human norovirus in human intestinal enteroids.

Science advances·2026

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Direct selection of human genomic loci by microarray hybridization.

Thomas J Albert¹, Michael N Molla, Donna M Muzny

¹NimbleGen Systems Inc., 1 Science Court, Madison, Wisconsin 53711, USA. talbert@nimblegen.com

|October 16, 2007

Summary

High-density microarrays efficiently enrich specific human genome sequences for high-throughput sequencing. This direct selection method surpasses multiplex PCR for large-scale genomic analysis.

More Related Videos

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Related Experiment Videos

Last Updated: Jul 10, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

High-throughput sequencing requires efficient methods for targeting specific genomic regions.
Previous methods like multiplex PCR have limitations for large-scale genomic analysis.

Purpose of the Study:

To evaluate the efficacy of high-density microarrays for enriching specific human genomic sequences.
To compare microarray-based enrichment with multiplex PCR for large-scale genomic region analysis.

Main Methods:

Application of high-density microarrays for capturing 6,726 exon segments (approx. 500 bases each).
Capture of locus-specific genomic regions (200 kb to 5 Mb).
Sequencing of captured targets using a 454 Life Sciences FLX sequencer.

Main Results:

The majority of sequence reads obtained directly corresponded to the targeted genomic regions.
Microarray-based enrichment demonstrated high specificity for both exon and locus-specific targets.
Successful sequencing of enriched targets was achieved.

Conclusions:

High-density microarrays provide an effective method for targeted enrichment of human genomic DNA.
This direct selection approach is superior to multiplex PCR for large-scale genomic analyses.
The method facilitates efficient high-throughput sequencing of selected genomic regions.