Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Thermal conductivity of the diamond-chain compound Cu₃(CO₃)₂(OH)₂.

Journal of physics. Condensed matter : an Institute of Physics journal·2016

Same author

Associations between HLA-A\B\DRB1 polymorphisms and risks of vulvar lichen sclerosus or squamous cell hyperplasia of the vulva.

Genetics and molecular research : GMR·2015

Same author

Role of survivin in the pathogenesis of papillary thyroid carcinoma.

Genetics and molecular research : GMR·2015

Same author

Human recognition memory and conflict control: An event-related potential study.

Neuroscience·2015

Same author

Carotid stenosis and cognitive impairment amongst older Chinese adults living in a rural area: a population-based study.

European journal of neurology·2015

Same author

Neurodegeneration in frontotemporal lobar degeneration and motor neurone disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.

Neuropathology and applied neurobiology·2015

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Patterning biomolecules with a water-soluble release and protection interlayer.

J Shi¹, X F Ni, Y Chen

¹Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.

Langmuir : the ACS Journal of Surfaces and Colloids

|October 16, 2007

Summary

This study introduces a novel lithography technique for precise biomolecule patterning. The method enhances biomolecule immobilization stability on substrates, outperforming existing microcontact printing methods.

More Related Videos

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

Related Experiment Videos

Last Updated: Jul 10, 2026

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

Area of Science:

Materials Science
Biotechnology
Surface Chemistry

Background:

Accurate patterning of biomolecules is crucial for advanced biological applications.
Existing methods like microcontact printing have limitations in stability and resolution.
Developing robust and high-resolution biomolecule immobilization techniques is an ongoing challenge.

Purpose of the Study:

To present a general lithography method for high-resolution biomolecule patterning.
To demonstrate enhanced stability of biomolecule immobilization compared to current techniques.
To showcase the application of this method using soft UV nanoimprint lithography.

Main Methods:

Utilizing a bilayer resist system for biomolecule patterning.
Immobilizing biomolecules on a substrate, followed by a water-soluble polymer interlayer.
Employing reactive ion etching for pattern transfer and subsequent resist removal in water.
Applying soft UV nanoimprint lithography for high-density dot array fabrication.

Main Results:

Achieved high-resolution patterning of poly-(L-lysine) molecules on a glass substrate.
Demonstrated improved stability of biomolecule immobilization.
Verified patterning through fluorescence imaging and cell proliferation assays.
Observed superior performance compared to microcontact printing techniques.

Conclusions:

The developed lithography method offers a versatile approach for high-resolution biomolecule patterning.
The bilayer resist system significantly enhances the stability of immobilized biomolecules.
This technique holds promise for applications requiring precise and stable biomolecular arrangements.