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Expression of TNF-alpha protein in omental and subcutaneous adipose tissue in obesity
Yan Li Cao1, Cui Zhu Hu, Xin Meng
1Department of Endocrinology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, Heping District, Shenyang, Liaoning 110001, China.
Background:
During the last several years the role of adipose tissue in contributing to obesity-associated cardiovascular and metabolic risk has gained much attention.
Aim:
To examine the expression of TNF-alpha protein in omental and subcutaneous adipose tissue in correlation with plasma PAI-1 and other clinical parameters in obesity subjects.
Material And Methods:
Paired biopsies of omental and subcutaneous fat were collected during surgery in 32 obesity subjects. The expression of TNF-alpha protein in omental and subcutaneous fat was quantified by using Western blot method, and correlations with plasma PAI-1, homeostasis model assessment insulin resistance (HOMA-IR) and lipid were investigated.
Results:
TNF-alpha protein expression was higher in omental than in subcutaneous adipose tissue (P<0.01). Significant positive linear correlations were found between TNF-alpha protein in omental adipose tissue and plasma PAI-1 in obesity subjects. TNF-alpha protein in omental fat was positively associated with HOMA-IR, triglycerides and negatively with HDL-cholesterol.
Conclusion:
TNF-alpha expression in omental adipose tissue could play a key role in contributing to cardiovascular risk in central obesity subjects.
