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Updated: Jul 10, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
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The toxicity study on marine low-temperature lysozyme.

Chun-ling Yan1, Bo-xiao Ding, Xiao-ming Lan

  • 1Qingdao University, Medical Collogy, Qingdao 266021, PR China.

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
|October 16, 2007
PubMed
Summary

Marine low-temperature lysozyme, active in cold conditions, exhibits broad-spectrum antibiotic properties. Toxicity studies in mice and rats indicate it is safe for use at tested experimental doses.

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Area of Science:

  • Biochemistry
  • Microbiology
  • Toxicology

Background:

  • Marine bacteria offer a source of novel enzymes with unique properties.
  • Lysozymes are enzymes with antimicrobial activity, crucial in innate immunity.
  • Low-temperature active enzymes have potential applications in various industries.

Purpose of the Study:

  • To characterize marine low-temperature lysozyme.
  • To evaluate the acute and subchronic toxicity of marine low-temperature lysozyme.

Main Methods:

  • Physicochemical properties (molecular weight, optimal pH/temperature) were determined.
  • Enzyme activity was assessed with various ions and chemical agents.
  • Acute toxicity (LD50) in mice and subchronic toxicity (90-day oral administration) in rats were evaluated.

Main Results:

  • Marine low-temperature lysozyme has a molecular weight of ~16 kD, optimal activity at pH 6.5 and 35°C.
  • Activity was enhanced by Zn(2+)/Cu(2+) and inhibited by Mn(2+)/Ag(+); it showed good compatibility with EDTA and KH2PO4.
  • The LD50 was 4530 mg/kg bw; no significant adverse effects on blood biochemistry or organ indices were observed in subchronic studies.

Conclusions:

  • Marine low-temperature lysozyme possesses favorable enzymatic characteristics and broad-spectrum antimicrobial activity.
  • The enzyme demonstrates a favorable safety profile with no significant toxicity observed in acute and subchronic animal studies.
  • These findings support the potential safe application of marine low-temperature lysozyme in relevant experimental contexts.