Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Flame Photometry: Lab

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Surgical Safety after Preoperative Chemotherapy for Perihilar Cholangiocarcinoma: Multi-Institutional Propensity-Matched Analysis.

Annals of surgical oncology·2026

Same author

Feasibility of Surgical Resection for Advanced Gallbladder Carcinoma Based on Preoperative Clinical T Stagings: A Multicenter Retrospective Analysis (SNOW-22-05).

Journal of hepato-biliary-pancreatic sciences·2026

Same author

Early clinical impact of mesenteric Kocherization in robotic pancreaticoduodenectomy: a propensity score-matched comparative study.

Surgical endoscopy·2026

Same author

Postoperative risk stratification after upfront surgery for resectable pancreatic ductal adenocarcinoma in the S-1 adjuvant era.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]·2026

Same author

Primary leiomyosarcoma of the portal vein: A progressively enhancing fusiform mass without biliary dilatation as a rare differential for hilar tumors.

Radiology case reports·2026

Same author

A Case of Mediastinitis Due to Fulminant Group A Streptococcal Infection.

Surgical case reports·2026

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

A chemiluminescence biochemical oxygen demand measuring method.

Hideaki Nakamura¹, Yuta Abe, Rui Koizumi

¹School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. nakamura@bs.teu.ac.jp

Analytica Chimica Acta

|October 16, 2007

Summary

A new chemiluminescence method accurately determines biochemical oxygen demand (BOD) using Baker's yeast and a luminol reaction. This rapid assay offers a sensitive detection limit for environmental water quality monitoring.

More Related Videos

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Related Experiment Videos

Last Updated: Jul 10, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Area of Science:

Analytical Chemistry
Environmental Science
Biochemistry

Background:

Traditional biochemical oxygen demand (BOD) methods are time-consuming.
There is a need for rapid and sensitive BOD determination techniques.
Chemiluminescence (CL) offers potential for sensitive analytical measurements.

Purpose of the Study:

To develop and characterize a novel chemiluminescence-based method for determining biochemical oxygen demand (BOD(CL)).
To utilize the redox reaction between quinone and Baker's yeast for BOD measurement.
To optimize and validate the new method for environmental water analysis.

Main Methods:

Employed luminol chemiluminescence catalyzed by ferricyanide with oxidized menadione and Saccharomyces cerevisiae.
Utilized a batch-type luminometer for measurements.
Optimized reaction conditions, including solvent (dimethyl sulfoxide), and assessed linearity, detection limits, and stability.

Main Results:

Achieved a linear response for hydrogen peroxide (0.1–100 µM) with high correlation (r²=0.9999).
Established a practical relationship for BOD(CL) over a range of 11–220 mg O2 L⁻¹ with a detection limit of 5.5 mg O2 L⁻¹ after a 5-min incubation.
Demonstrated method characterization through testing organic substances, ion interference, and real river water samples.

Conclusions:

The developed BOD(CL) method is rapid, sensitive, and suitable for environmental water quality assessment.
The yeast suspension showed stability for up to 8 days at 4°C, with some reduction in response.
Further research could explore extending the stability or improving the long-term reliability of the yeast reagent.