Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Role of Myosin in Cell Migration

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...

Cytoskeletal Coordination in Cell Migration

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

Smooth Muscle Contraction

Smooth Muscle Contraction

Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...

Cell Migration

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

Cell Migration

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.

Cell Polarization by Rho Proteins

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reply to the Letter to the Editor from Yanyu Zhang: 'Considering a renal origin of endocrine hypertension in ASIC1a-deficient mice'.

The Journal of physiology·2026

Same author

Mitochondrial acid-sensing ion channel 1a deficiency induces mitochondrial dysfunction in pulmonary arterial smooth muscle cells.

American journal of physiology. Lung cellular and molecular physiology·2026

Same author

Loss of βENaC Prevents Hepatic Steatosis but Promotes Abdominal Fat Deposition Associated with a High-Fat Diet.

Biology·2025

Same author

Acid-sensing ion channel 1a deficiency drives endocrine hypertension in male mice.

The Journal of physiology·2025

Same author

Mitochondrial acid-sensing ion channel 1a deficiency induces mitochondrial dysfunction in pulmonary arterial smooth muscle cells.

bioRxiv : the preprint server for biology·2025

Same author

The impact of Asic2 deletion on metabolic homeostasis in mice.

Physiological reports·2025

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

ASIC proteins regulate smooth muscle cell migration.

Samira C Grifoni¹, Nikki L Jernigan, Gina Hamilton

¹Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216, USA.

Microvascular Research

|October 16, 2007

Summary

This summary is machine-generated.

Acid Sensing Ion Channels (ASICs) are crucial for vascular smooth muscle cell migration. This study found that ASIC1, ASIC2, and ASIC3 expression is necessary for normal cell migration and wound healing.

More Related Videos

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

Related Experiment Videos

Last Updated: Jul 10, 2026

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

Area of Science:

Cell Biology
Ion Channel Physiology
Vascular Biology

Background:

Epithelial Sodium Channels (ENaC) are known to be essential for vascular smooth muscle cell (VSMC) migration.
The role of closely related Acid Sensing Ion Channel (ASIC) proteins in VSMC migration remains uninvestigated.

Purpose of the Study:

To investigate the expression and functional significance of ASIC proteins in cellular migration.
To determine if ASIC proteins play a role in vascular smooth muscle cell migration.

Main Methods:

RT-PCR and immunolabeling were used to detect ASIC1, ASIC2, ASIC3, and ASIC4 expression in A10 cells.
Small interference RNA (siRNA) was employed to silence individual ASIC expression.
Wound healing and PDGF-bb-initiated chemotactic migration assays were performed to assess the functional impact of ASIC suppression.

Main Results:

ASIC1, ASIC2, and ASIC3, but not ASIC4, were expressed in A10 cells.
siRNA-mediated suppression significantly reduced ASIC1 (63%), ASIC2 (44%), and ASIC3 (55%) expression.
Suppression of ASIC1, ASIC2, and ASIC3 inhibited wound healing by 10%, 20%, and 26%, respectively.
ASIC1 and ASIC3 suppression inhibited chemotactic migration by 30% and 45%, while ASIC2 suppression led to a 4% increase.

Conclusions:

ASIC protein expression is required for normal cellular migration.
ASIC proteins may represent a novel target for modulating cellular migration processes.