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Updated: Jul 10, 2026
Processing the Loblolly Pine PtGen2 cDNA Microarray
Published on: March 20, 2009
Identification of genes differentially expressed during adventitious shoot induction in Pinus pinea cotyledons by
Pablo Alonso1, Millán Cortizo, Francisco R Cantón
1Area de Fisiología Vegetal, Departamento BOS, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, E-33071, Oviedo, Spain.
Abstract:
As part of a study aimed at understanding the physiological and molecular mechanisms involved in adventitious shoot bud formation in pine cotyledons, we conducted a transcriptome analysis to identify early-induced genes during the first phases of adventitious caulogenesis in Pinus pinea L. cotyledons cultured in the presence of benzyladenine. A subtractive cDNA library with more than 700 clones was constructed. Of these clones, 393 were sequenced, analyzed and grouped according to their putative function. Quantitative real-time PCR analysis was performed to confirm the differential expression of 30 candidate genes. Results are contrasted with available data for other species.
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