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Updated: Jul 10, 2026

Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Published on: August 6, 2008
Interference of a four-hole aperture for on-chip quantitative two-dimensional differential phase imaging
Matthew Lew1, Xiquan Cui, Xin Heng
1Department of Electrical Engineering, California Institute of Technology, MC 136-93, Pasadena, California 91125, USA. mlew@its.caltech.edu
Abstract:
We present a novel on-chip method for quantitative two-dimensional differential phase imaging. This technique uses four circular holes (600 nm diameter, 1.2 mum spacing) arranged in a 'plus' pattern that are fabricated in a layer of metal above a complementary metal-oxide semiconductor (CMOS) imaging sensor. The interference pattern of the aperture shifts position with respect to the differential phase of the incident light. By imaging the interference pattern with the CMOS sensor, this method measures amplitude and differential phase (1 degrees /microm sensitivity for signal-to-noise ratio >/=16 dB) of the incident light field simultaneously. An application to optical beam profiling is presented; we show the amplitude and differential phase profiles of a Gaussian laser beam and an optical vortex.
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