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Stem Cell Niche

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

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Related Experiment Video

Updated: Jul 10, 2026

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

The intestinal stem cell niche studied through conditional transgenesis.

A R Clarke¹, V Meniel

¹Cardiff School of Biosciences, Cardiff University, Wales CF10 3US Cardiff, UK. clarkear@cardiff.ac.uk

Ernst Schering Foundation Symposium Proceedings

|October 18, 2007

Summary

Intestinal stem cell regeneration mechanisms are poorly understood. This review explores stem cell maintenance and repopulation, highlighting the Wnt pathway

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Last Updated: Jul 10, 2026

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

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Published on: April 1, 2021

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

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Published on: October 11, 2022

The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells

The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells

Published on: November 20, 2017

Area of Science:

Gastroenterology and Regenerative Medicine
Stem Cell Biology
Molecular Biology

Background:

Intestinal stem cell maintenance and regeneration mechanisms are not fully understood.
Injury to intestinal crypt stem cells necessitates repopulation from unknown sources.
Understanding these processes is crucial for addressing malignant diseases and developing regenerative therapies for conditions like Crohn's disease.

Purpose of the Study:

To review basic mechanisms regulating intestinal stem cell viability and maintenance.
To summarize recent findings on the Wnt pathway's role in intestinal stem cell regulation.
To provide insights into stem cell plasticity and its contribution to intestinal diseases.

Main Methods:

Literature review of existing experimental data on intestinal stem cell biology.
Summary of laboratory's recent research findings.
Focus on the Wnt signaling pathway's involvement.

Main Results:

The Wnt pathway is essential for intestinal stem cell maintenance and regeneration.
Experimental data suggests specific genetic elements influence stem cell fate.
Repopulation mechanisms following injury remain an area requiring further investigation.

Conclusions:

Further research into intestinal stem cell regulation is vital for therapeutic development.
The Wnt pathway plays a critical role in maintaining intestinal epithelial homeostasis.
Elucidating stem cell behavior can inform treatments for inflammatory bowel disease and radiation injury.