Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Germanium-embedded bioactive fabric reduces bacterial bioburden and modulates fibroblast and macrophage behavior <i>in vitro</i>.

Frontiers in bioengineering and biotechnology·2026
Same author

Local sinonasal and regional nodal effects of lymph node irradiation in a pilot study of dogs with naturally occurring sinonasal tumors.

International journal of radiation oncology, biology, physics·2026
Same author

Intra-articular 2.5% polyacrylamide hydrogel alters synovial immune pathways in equine experimental osteoarthritis.

American journal of veterinary research·2026
Same author

Synovial transcriptomic biomarkers predict structural severity in equine osteochondral fragment-induced experimental osteoarthritis.

American journal of veterinary research·2026
Same author

Diagnostic Investigation of Two Cases of Oslerus osleri Infection in Dogs: Microscopic Challenges and Molecular Characterisation.

Veterinary medicine and science·2026
Same author

Toll-like receptor 3-activated allogeneic umbilical cord blood-derived mesenchymal stromal cells as an adjunct for the treatment of synovial sepsis in 3 horses.

Journal of the American Veterinary Medical Association·2026

Related Experiment Video

Updated: Jul 10, 2026

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
09:45

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

Published on: August 24, 2018

Neutrophil function in septic dogs.

Craig Webb1, Kelly McCord, Steven Dow

  • 1Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft Collins, CO 80523, USA. cbwebb@colostate.edu

Journal of Veterinary Internal Medicine
|October 18, 2007
PubMed
Summary
This summary is machine-generated.

Septic dogs exhibit impaired neutrophil function, specifically a diminished oxidative burst, potentially hindering bacterial clearance. This study investigated neutrophil dysfunction in canine sepsis.

More Related Videos

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
06:45

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

Published on: November 21, 2016

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
06:48

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jul 10, 2026

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
09:45

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

Published on: August 24, 2018

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
06:45

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

Published on: November 21, 2016

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
06:48

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Published on: January 7, 2019

Area of Science:

  • Veterinary Immunology
  • Canine Sepsis Research
  • Neutrophil Function Studies

Background:

  • Leukocyte hypo-inflammatory states are observed in human sepsis.
  • Canine sepsis is associated with impaired neutrophil function, a hypo-inflammatory state.

Purpose of the Study:

  • To assess neutrophil dysfunction in dogs with sepsis.
  • To compare neutrophil function in septic and healthy dogs.

Main Methods:

  • Flow cytometry was used to quantify the phagolysosomal oxidative burst and Fc receptor-mediated phagocytosis of Escherichia coli.
  • Intracellular reduced glutathione levels were measured as an indicator of oxidative stress.

Main Results:

  • Septic dogs showed a significantly lower phagolysosomal oxidative burst in neutrophils compared to healthy controls (p = 0.001).
  • Neutrophil phagocytosis of opsonized E. coli was significantly increased in septic dogs (p = 0.004).
  • No significant difference in intracellular reduced glutathione was found between septic and healthy dogs.

Conclusions:

  • Septic dogs display decreased neutrophil function, characterized by a diminished oxidative burst.
  • This impaired neutrophil function may affect the innate immune system's ability to combat bacterial infections in septic dogs.