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Updated: Jul 10, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Published on: August 4, 2009
Chemiluminescent probe for the in vitro detection of protease activity
Jean-Alexandre Richard1, Ludovic Jean, Anthony Romieu
1Equipe de Chimie Bio-Organique, Université de Rouen, INSA de Rouen, CNRS UMR 6014, COBRA, IRCOF, 1 rue Lucien Tesnière, Mont-Saint-Aignan Cedex, France
Abstract:
A strategy involving the use of a self-immolative linker has been investigated for the chemiluminescent sensing of proteases. The reactive linker enabled the release of a 1,2-dioxetane light precursor. As a proof of principle, caspase-3, a key peptidase involved in apoptosis has been targeted. An in vitro assay has been carried out and proved the decomposition of the linker and the selectivity for caspase-3.
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