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Related Experiment Video

Updated: Jul 10, 2026

A Novel Approach to Assess Motor Outcome of Deep Brain Stimulation Effects in the Hemiparkinsonian Rat: Staircase and Cylinder Test
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A Novel Approach to Assess Motor Outcome of Deep Brain Stimulation Effects in the Hemiparkinsonian Rat: Staircase and Cylinder Test

Published on: May 31, 2016

Neurotoxicity screening test for deep brain stimulation leads.

Sandy Hooper1, Tracy Cameron

  • 1Advanced Neuromodulation Systems Inc., 6901 Preston Road, Plano, TX 75024, USA. s.hooper@ans-medical.com

Journal of Biomaterials Science. Polymer Edition
|October 18, 2007
PubMed
Summary

This study introduces an in vitro human cell testing method to assess neurotoxicity of deep brain stimulation leads, reducing reliance on animal models. This approach offers a practical and efficient alternative for preclinical safety screening of medical devices.

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Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Toxicology

Background:

  • Preclinical neurotoxicity testing for implantable medical devices traditionally relies on costly and time-consuming animal studies.
  • There is a need for more efficient and ethical methods to evaluate the safety of medical devices like deep brain stimulation leads.

Purpose of the Study:

  • To develop and validate an in vitro human cell-based testing process for screening deep brain stimulation (DBS) leads for neurotoxicity.
  • To compare the efficacy of this in vitro method against traditional animal testing protocols.

Main Methods:

  • Utilized qualitative and quantitative cell-culture studies with human cerebellar, cortical, and glial cell lines.
  • Extracted materials from a deep brain stimulation lead in saline and minimum essential medium under specified conditions.

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Last Updated: Jul 10, 2026

A Novel Approach to Assess Motor Outcome of Deep Brain Stimulation Effects in the Hemiparkinsonian Rat: Staircase and Cylinder Test
07:14

A Novel Approach to Assess Motor Outcome of Deep Brain Stimulation Effects in the Hemiparkinsonian Rat: Staircase and Cylinder Test

Published on: May 31, 2016

Targeting Neuronal Fiber Tracts for Deep Brain Stimulation Therapy Using Interactive, Patient-Specific Models
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  • Performed cytotoxicity and cell proliferation assays.

    • Main Results:

    • Positive controls (Tygon F-4040-A) demonstrated severe cytotoxicity across all tested cell lines.
    • Negative controls (high-density polyethylene) and the lead extraction media showed non-cytotoxic results in all cell lines.
    • The in vitro human cell models effectively differentiated between cytotoxic and non-cytotoxic materials.

    Conclusions:

    • In vitro human neuronal cell-specific cytotoxicity testing, combined with ISO-10993 standards, provides an efficient and practical method for preclinical safety screening of medical devices.
    • This approach can reduce the need for extensive animal testing, saving time and resources while decreasing animal usage.