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Additional microsatellite markers for mouse genome mapping.

C M Hearne1, M A McAleer, J M Love

  • 1Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UK.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|January 1, 1991
PubMed
Summary
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Researchers analyzed mouse microsatellites, identifying numerous genetic markers. These microsatellites are valuable for constructing a detailed genetic map of the mouse genome.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Microsatellites, or simple repetitive DNA elements, are crucial for genetic mapping.
  • Analyzing microsatellite variation across mouse strains aids in understanding genome structure and diversity.

Purpose of the Study:

  • To identify and characterize microsatellites in the mouse genome.
  • To assess the variability of these microsatellites between different mouse strains.
  • To evaluate their utility as single-locus markers for genetic mapping.

Main Methods:

  • Bioinformatic analysis of mouse sequence data from EMBL and GenBank.
  • Polymerase chain reaction (PCR) amplification of microsatellites.
  • Sizing of PCR products using gel electrophoresis.

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  • Primer design from unique flanking sequences.

    • Main Results:

    • Analysis of 185 microsatellites, with 85% exhibiting allelic variation.
    • Significant length variation observed between common laboratory mouse strains (C57BL/6J, DBA/2J) and wild mice (Mus spretus).
    • 74 new microsatellites were characterized, adding to the existing dataset.

    Conclusions:

    • Microsatellites are abundant and highly polymorphic in the mouse genome.
    • These microsatellites serve as informative single-locus markers.
    • They are essential tools for linkage analysis in constructing a high-resolution mouse genome map.