Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 10, 2026

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

Dextran synthesis by immobilized dextran sucrase.

A Lopez1, P Monsan

  • 1Laboratoire de Génie Biochimique, Institut National des Sciences Appliquées, Toulouse.

Biochimie
|January 1, 1980
PubMed
Summary

Researchers optimized dextran sucrase production and immobilization. Immobilized dextran sucrase showed high activity, with support properties influencing efficiency and kinetics for improved enzyme applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Indolent systemic mast cell disease in adults: immunophenotypic characterization of bone marrow mast cells and its diagnostic implications.

Blood·1998
Same author

Evaluation of the toxicity of guarana with in vitro bioassays.

Ecotoxicology and environmental safety·1998
Same author

Skin necrosis at the injection site induced by low-molecular-weight heparin: case report and review.

Dermatology (Basel, Switzerland)·1998
Same author

Severe hemolytic anemia due to multiple red cell alloantibodies after an ABO-incompatible allogeneic bone marrow transplant.

Transfusion·1998
Same author

Rectocele is associated with paradoxical anal sphincter reaction.

International journal of colorectal disease·1998
Same author

Prolonged pneumoperitoneum at 15 mmHg causes lactic acidosis.

Surgical endoscopy·1998

Area of Science:

  • Biotechnology
  • Enzyme Engineering

Background:

  • Dextran sucrase is an enzyme with various industrial applications.
  • Optimizing its production and immobilization is crucial for efficient use.

Purpose of the Study:

  • To enhance dextran sucrase production from *Leuconostoc mesenteroides*.
  • To immobilize the enzyme onto a silica support and characterize its properties.
  • To investigate factors affecting immobilized enzyme performance.

Main Methods:

  • Fermentation of *Leuconostoc mesenteroides* NRRL B-512.
  • Enzyme purification using ultrafiltration and gel permeation chromatography.
  • Immobilization onto activated amino porous silica (Spherosil) via covalent coupling.
  • Characterization of immobilized enzyme activity and kinetics.

Main Results:

  • Dextran sucrase production was improved through optimized fermentation.
  • Calcium chloride addition significantly enhanced specific enzyme activity.
  • Immobilized dextran sucrase achieved high activity (up to 830 units/g support).
  • Support surface area and intraparticular diffusion affected coupling efficiency and kinetics.

Conclusions:

  • Optimized fermentation and purification yielded active dextran sucrase.
  • Covalent immobilization on silica supports provides a stable and active enzyme preparation.
  • Understanding support properties is key to maximizing immobilized enzyme performance for industrial applications.

More Related Videos

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
07:20

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)

Published on: June 14, 2021

Related Experiment Videos

Last Updated: Jul 10, 2026

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
07:20

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)

Published on: June 14, 2021