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Reciprocal endoderm-mesoderm interactions mediated by fgf24 and fgf10 govern pancreas development.
Isabelle Manfroid1, François Delporte, Ariane Baudhuin
1GIGA-Research-Unité de Biologie Moléculaire et Génie Génétique, Tour B34, Université de Liège, B-4000 Sart Tilman, Belgium. isabelle.manfroid@ulg.ac.be
Development (Cambridge, England)
|October 19, 2007
Summary
Zebrafish endoderm signals FGF24 to pattern adjacent mesoderm. FGF10 and FGF24 redundantly specify the ventral pancreas, crucial for exocrine tissue development.
Area of Science:
- Developmental Biology
- Endocrinology
- Genetics
Background:
- Pancreatic mesenchyme is vital for pancreatic epithelium growth in amniotes, mediated by fibroblast growth factors.
- Factors governing pancreatic mesenchyme formation remain largely uncharacterized.
Purpose of the Study:
- To identify factors involved in pancreatic mesenchyme formation in zebrafish.
- To elucidate the roles of endoderm-mesoderm signaling in ventral pancreas development.
Main Methods:
- Characterization of pancreatic lateral plate mesoderm in zebrafish embryos.
- Analysis of gene expression patterns (isl1, fgf10, meis, fgf24).
- Functional studies on Fgf10 and Fgf24 in ventral pancreas specification.
Main Results:
- Zebrafish endoderm expresses fgf24, initiating pancreatic lateral plate mesoderm patterning.
- The pancreatic lateral plate mesoderm is analogous to murine pancreatic mesenchyme based on gene expression.
- Fgf10 and Fgf24 act redundantly and are essential for ventral pancreas specification.
Conclusions:
- Sequential endoderm-mesoderm signaling is critical for ventral pancreas specification and growth.
- This signaling explains the origin of the entire exocrine tissue from the zebrafish ventral pancreatic bud.
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