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Comparison of multiple-locus variable-number tandem-repeat analysis with other PCR-based methods for typing Brucella
David García-Yoldi1, Philippe Le Fleche, María J De Miguel
1Departamento de Microbiología y Parasitología, Universidad de Navarra, c/ Irunlarrea no 1, 31008, Pamplona, Spain.
Journal of Clinical Microbiology
|October 19, 2007
Summary
Multiple-locus variable-number tandem-repeat analysis (MLVA) effectively identified Brucella suis outbreaks and confirmed wildlife as a reservoir for zoonotic brucellosis, outperforming other molecular typing methods.
Area of Science:
- Veterinary Microbiology
- Molecular Epidemiology
- Infectious Diseases
Background:
- Brucella suis causes brucellosis, a zoonotic disease with significant public health and economic impacts.
- Accurate molecular typing is crucial for tracking disease transmission and identifying reservoirs.
Purpose of the Study:
- To compare the efficacy of Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism (PCR-RFLP) for Brucella suis typing.
- To determine the most suitable method for identifying brucellosis outbreaks and confirming wildlife reservoirs.
Main Methods:
- Comparative analysis of three molecular typing techniques: MLVA, multiplex PCR, and PCR-RFLP.
- Application of these methods to Brucella suis isolates for strain differentiation.
Main Results:
- All molecular assays demonstrated perfect concordance in typing Brucella suis isolates.
- MLVA was uniquely capable of identifying brucellosis outbreaks.
- MLVA confirmed the role of wildlife as a reservoir for zoonotic brucellosis.
Conclusions:
- MLVA is a superior molecular typing tool for Brucella suis surveillance.
- MLVA facilitates the detection of disease outbreaks and the identification of zoonotic reservoirs in wildlife populations.
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